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13999 1 ap  (Proteintech)


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    Structured Review

    Proteintech 13999 1 ap
    13999 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13999+1+ap/OLIG2+Antibody/pmc13045046-21-5-3
    Average 96 stars, based on 167 article reviews
    13999 1 ap - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:


    Article Title: Molecular Features of the Measles Virus Viral Fusion Complex That Favor Infection and Spread in the Brain
    Article Snippet: Rabbit polyclonal anti-Olig2; Proteintech , No. 13999-1-AP.

    Article Title: Myelin Damage in Diffuse Axonal Injury
    Article Snippet: Olig2 , Anti-rabbit , Mature OLs and OPCs , 13999-1-AP, Proteintech, Rosemont, IL, United States.

    Immunohistochemistry:

    Article Title: Maternal obesity alters histone modifications mediated by the interaction between EZH2 and AMPK, impairing neural differentiation in the developing embryonic brain cortex
    Article Snippet: NKX2-2 Rabbit pAb , ABclonal , A16696 , Immunohistochemistry, Western blotting. .. OLIG2 antibody , Proteintech , 13999-1-AP , Immunohistochemistry, Western blotting. .. Purified anti-MBP antibody , Biolegend , 836504 , Immunohistochemistry, Western blotting.

    Western Blot:

    Article Title: Maternal obesity alters histone modifications mediated by the interaction between EZH2 and AMPK, impairing neural differentiation in the developing embryonic brain cortex
    Article Snippet: NKX2-2 Rabbit pAb , ABclonal , A16696 , Immunohistochemistry, Western blotting. .. OLIG2 antibody , Proteintech , 13999-1-AP , Immunohistochemistry, Western blotting. .. Purified anti-MBP antibody , Biolegend , 836504 , Immunohistochemistry, Western blotting.

    Blocking Assay:

    Article Title: Sex-dependent impairments in oligodendrocyte maturation and myelination in offspring mice exposed to preeclampsia.
    Article Snippet: Preeclampsia, a prevalent obstetric complication with systemic implications for maternal and fetal health, has been increasingly linked to altered neurodevelopment and long-term neuropsychological sequelae in offspring.. While existing evidence highlights its potential to disrupt brain development, the sex-specific effects of preeclampsia on white matter development and oligodendrocyte maturation remain poorly characterized.. This study focused on assessing the effects of preeclampsia on offspring oligodendrocyte maturation, myelination and neurobehavioral outcomes.

    Incubation:

    Article Title: Sex-dependent impairments in oligodendrocyte maturation and myelination in offspring mice exposed to preeclampsia.
    Article Snippet: Preeclampsia, a prevalent obstetric complication with systemic implications for maternal and fetal health, has been increasingly linked to altered neurodevelopment and long-term neuropsychological sequelae in offspring.. While existing evidence highlights its potential to disrupt brain development, the sex-specific effects of preeclampsia on white matter development and oligodendrocyte maturation remain poorly characterized.. This study focused on assessing the effects of preeclampsia on offspring oligodendrocyte maturation, myelination and neurobehavioral outcomes.



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    Aurkb deficiency in microglia disrupts remyelination and oligodendrocyte density in the CPZ-induced demyelination model (A) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl mice (2–3 months old) were i.p. injected with TAM for 5 consecutive days. After one month, the mice were fed a 0.25% CPZ-enriched diet for 5 weeks to induce demyelination (CPZ group) or followed by a CPZ-free normal diet for an additional 2 weeks (Recovery group). The corpus callosum (CC) was analyzed by immunofluorescence (IF) and Black-Gold II myelin staining. (B and C) Representative Black-Gold II myelin staining images and quantification of myelin intensity ( n = 5 mice per genotype per group, Scale bars: 200 μm). (D–F) Representative immunofluorescence analysis and quantification of total Mbp and oligodendrocytes <t>(Olig2</t> + ), and g-h) representative immunofluorescence analysis and quantification of mature oligodendrocytes (CC-1 + ) ( n = 5 mice per genotype per group, Scale bars: 50 μm). Data are presented as the mean ± SD. two-way ANOVA with Bonferroni multiple comparisons test in (C, E, F, and H); ∗∗ p < 0.01 and ∗∗∗ p < 0.001; ns, not significant compared with the Aurkb fl/fl group.
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    Image Search Results


    Aurkb deficiency in microglia disrupts remyelination and oligodendrocyte density in the CPZ-induced demyelination model (A) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl mice (2–3 months old) were i.p. injected with TAM for 5 consecutive days. After one month, the mice were fed a 0.25% CPZ-enriched diet for 5 weeks to induce demyelination (CPZ group) or followed by a CPZ-free normal diet for an additional 2 weeks (Recovery group). The corpus callosum (CC) was analyzed by immunofluorescence (IF) and Black-Gold II myelin staining. (B and C) Representative Black-Gold II myelin staining images and quantification of myelin intensity ( n = 5 mice per genotype per group, Scale bars: 200 μm). (D–F) Representative immunofluorescence analysis and quantification of total Mbp and oligodendrocytes (Olig2 + ), and g-h) representative immunofluorescence analysis and quantification of mature oligodendrocytes (CC-1 + ) ( n = 5 mice per genotype per group, Scale bars: 50 μm). Data are presented as the mean ± SD. two-way ANOVA with Bonferroni multiple comparisons test in (C, E, F, and H); ∗∗ p < 0.01 and ∗∗∗ p < 0.001; ns, not significant compared with the Aurkb fl/fl group.

    Journal: iScience

    Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

    doi: 10.1016/j.isci.2026.114718

    Figure Lengend Snippet: Aurkb deficiency in microglia disrupts remyelination and oligodendrocyte density in the CPZ-induced demyelination model (A) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl mice (2–3 months old) were i.p. injected with TAM for 5 consecutive days. After one month, the mice were fed a 0.25% CPZ-enriched diet for 5 weeks to induce demyelination (CPZ group) or followed by a CPZ-free normal diet for an additional 2 weeks (Recovery group). The corpus callosum (CC) was analyzed by immunofluorescence (IF) and Black-Gold II myelin staining. (B and C) Representative Black-Gold II myelin staining images and quantification of myelin intensity ( n = 5 mice per genotype per group, Scale bars: 200 μm). (D–F) Representative immunofluorescence analysis and quantification of total Mbp and oligodendrocytes (Olig2 + ), and g-h) representative immunofluorescence analysis and quantification of mature oligodendrocytes (CC-1 + ) ( n = 5 mice per genotype per group, Scale bars: 50 μm). Data are presented as the mean ± SD. two-way ANOVA with Bonferroni multiple comparisons test in (C, E, F, and H); ∗∗ p < 0.01 and ∗∗∗ p < 0.001; ns, not significant compared with the Aurkb fl/fl group.

    Article Snippet: The following primary antibodies were used: rabbit anti-Iba-1 antibody (1:500, Wako, Cat: 019–19741), mouse anti-Iba-1 antibody (1:400, Abcam, Cat: ab283319), rat anti-CD206 antibody (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU antibody (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3 (Ser10) antibody (1:200, Cell Signaling Technology, Cat: 9706S), mouse anti-APC (1:100, CC-1, Merck, Cat: OP80), rat anti-myelin basic protein (Mbp) monoclonal antibody (1:500, Abcam, Cat: ab7349), rabbit anti-degraded myelin basic protein (dMbp) antibody (1:2000, Millipore Sigma, Cat: AB5864), rabbit anti-Olig2 antibody (1:500, Proteintech, Cat: 13999-1-AP) and rat anti-CD68 antibody (1:500, Abcam, Cat: ab53444).

    Techniques: Injection, Immunofluorescence, Staining